| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 731 | Bacillus smithii | AAATCGTGCGCTCGTCGT | ACGTGTCTTGGCAAGCGT | 60.43 | 60.20 | 271 | 79 |
100.00%
|
100.00%
|
| Primer 732 | Bacillus smithii | AGCGTGTCTGGATTGCCA | TCGGCAAATCTGGCTGCA | 59.25 | 59.97 | 187 | 79 |
100.00%
|
100.00%
|
| Primer 733 | Bacillus smithii | ACGCTGCAAAACAAGCCG | GGACGGTTCCGGATTGTGT | 59.97 | 60.00 | 213 | 79 |
100.00%
|
100.00%
|
| Primer 734 | Bacillus smithii | TGCTGCTGCCTTCTTCGT | GGCATGCAAGAGCGCAAT | 59.57 | 59.51 | 181 | 79 |
100.00%
|
100.00%
|
| Primer 735 | Bacillus smithii | ACCAAGCCATGAATTGCGG | CCGGTTCTTTTGCAGCGT | 59.11 | 58.97 | 173 | 79 |
100.00%
|
100.00%
|
| Primer 736 | Bacillus smithii | AGTGCGGTCGATGCGAAA | CCTCGCTTGTCAAGGACGT | 60.05 | 60.01 | 253 | 79 |
100.00%
|
100.00%
|
| Primer 737 | Bacillus smithii | TGCCAAAGCTCAGCCGAA | GCTTTGTCGCGAGCCATT | 59.89 | 59.13 | 234 | 79 |
100.00%
|
100.00%
|
| Primer 738 | Bacillus smithii | AGATCATCGCTGCGACCA | ATCCGGACGCAAAACCGT | 58.79 | 59.97 | 299 | 79 |
100.00%
|
100.00%
|
| Primer 739 | Bacillus smithii | AGGCCATTGACTTCGCCA | AACTTGCCAAGGCCATGG | 59.24 | 58.20 | 212 | 79 |
100.00%
|
100.00%
|
| Primer 740 | Bacillus smithii | CAACGAATTGGCAGCGCA | TTCCCAGCCTTTCAGCCA | 59.75 | 58.74 | 278 | 79 |
100.00%
|
100.00%
|